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3a3 n2 fluidigm  (fluidigm)


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    fluidigm 3a3 n2 fluidigm
    3a3 N2 Fluidigm, supplied by fluidigm, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3a3+n2+fluidigm/Anti-Human+IL-21+(3A3-N2)-172Yb/pm31607651-275-25-26
    Average 91 stars, based on 3 article reviews
    3a3 n2 fluidigm - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Recombinase Polymerase Amplification:

    Article Title: Maturation of the Human Intestinal Immune System Occurs Early in Fetal Development.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CD11b-167Er; ICRF44 Fluidigm Cat #3167011B; RRID:AB_2810974 IL-17a-169Tm; BL168* BioLegend Cat #512331; RRID:AB_2563779 IL-8-170Er; 6217* R&D Systems Cat #MAB208; RRID:AB_2249110 IL-21-172Yb; 3A3-N2 Fluidigm Cat #3172011B; RRID:AB_2810975 IL-10-176Yb; JES3-19F1* BioLegend Cat #506802; RRID:AB_315452 CCR7-BV421; 150503 BD Biosciences Cat #562555; RRID:AB_2728119 CD3-PE-Cy7; SK7 BioLegend Cat #344816; RRID:AB_10640737 CD4-APC-H7; RPA-T4 BD Biosciences Cat #560158; RRID:AB_1645478 CD8-PerCP-Cy5.5; RPA-T8 eBioscience Cat #45-0088-41; RRID:AB_1582256 CD45RA-FITC; HI100 BD Biosciences Cat #555488; RRID:AB_395879 CD45-BV785; HI30 BioLegend Cat #304048; RRID:AB_2563129 CD69-PE; FN50 BioLegend Cat #310906; RRID:AB_314841 Biological Samples Pitt Biospecimen Core University of Pittsburgh http://www.pittbiospecimencore.pitt.edu/ Chemicals, Peptides, and Recombinant Proteins Low-Barium PBS Sigma-Aldrich Cat #D8357 Bovine Serum Albumin, Protease-Free Sigma-Aldrich Cat #A3059 Sodium Azide Sigma-Aldrich Cat #71289 Cell-ID Intercalator-103Rh Fluidigm Cat #201103A Dimethyl Sulfoxide (DMSO) Fisher Scientific Cat #BP231-1; CAS:67-68-5 RPMI (1X) Media Gibco Cat #11875-093 GlutaMAX (100X) Gibco Cat #35050-061 HEPES (1M) Gibco Cat #15630-080 MEM NEAA (100X) Gibco Cat #11140-050 Na Pyruvate (100mM) Gibco Cat #11360-070 Penicillin/Streptomycin Gibco Cat #15140-122 Golgi Stop BD Biosciences Cat #554724 Golgi Plug BD Biosciences Cat #555029 Human Trustain FcX BioLegend Cat #422302 Cell Acquisition Solution Fluidigm Cat #201240 CD69 RNAScope Probe on Channel 2 ACD Bio Cat # 494471-C2 CD3 RNAScope Probe on Channel 1 ACD Bio Cat #553971-C1 Ionomycin Calcium Salt from Streptomyces conglobatus Sigma-Aldrich Cat #I0634 Phorbol 12-Myristate 13-Acetate (PMA) Sigma-Aldrich Cat #P8139 FoxP3 Fix and Perm Buffer A Thermo Fisher Scientific Cat #00-5123-43 FoxP3 Fix and Perm Buffer B Thermo Fisher Scientific Cat #00-5223-56 FoxP3 Wash Buffer Thermo Fisher Scientific Cat #A24261 Formaldehyde Solution Sigma-Aldrich Cat #F8775 DNase I Roche Cat #11284932001 Collagenase A Sigma-Aldrich Cat #10103586001 Fetal Bovine Serum Corning Cat #35-011-CV Cell-ID Intercalator-Ir Fluidigm Cat #201192A Maxpar Fix and Perm Buffer Fluidigm Cat #201067 Critical Commercial Assays RNAscope 2.5 HD Duplex Reagent Kit Advanced Cell Diagnostics Cat #322430 Wizard Genomic DNA Purification Kit Promega Cat #A1120 (Continued on next page) Developmental Cell 51, 1–17.e1–e5, November 4, 2019 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER CD11b-167Er; ICRF44 Fluidigm Cat #3167011B; RRID:AB_2810974 IL-17a-169Tm; BL168* BioLegend Cat #512331; RRID:AB_2563779 IL-8-170Er; 6217* R&D Systems Cat #MAB208; RRID:AB_2249110 IL-21-172Yb; 3A3-N2 Fluidigm Cat #3172011B; RRID:AB_2810975 IL-10-176Yb; JES3-19F1* BioLegend Cat #506802; RRID:AB_315452 CCR7-BV421; 150503 BD Biosciences Cat #562555; RRID:AB_2728119 CD3-PE-Cy7; SK7 BioLegend Cat #344816; RRID:AB_10640737 CD4-APC-H7; RPA-T4 BD Biosciences Cat #560158; RRID:AB_1645478 CD8-PerCP-Cy5.5; RPA-T8 eBioscience Cat #45-0088-41; RRID:AB_1582256 CD45RA-FITC; HI100 BD Biosciences Cat #555488; RRID:AB_395879 CD45-BV785; HI30 BioLegend Cat #304048; RRID:AB_2563129 CD69-PE; FN50 BioLegend Cat #310906; RRID:AB_314841 Biological Samples Pitt Biospecimen Core University of Pittsburgh http://www.pittbiospecimencore.pitt.edu/ Chemicals, Peptides, and Recombinant Proteins Low-Barium PBS Sigma-Aldrich Cat #D8357 Bovine Serum Albumin, Protease-Free Sigma-Aldrich Cat #A3059 Sodium Azide Sigma-Aldrich Cat #71289 Cell-ID Intercalator-103Rh Fluidigm Cat #201103A Dimethyl Sulfoxide (DMSO) Fisher Scientific Cat #BP231-1; CAS:67-68-5 RPMI (1X) Media Gibco Cat #11875-093 GlutaMAX (100X) Gibco Cat #35050-061 HEPES (1M) Gibco Cat #15630-080 MEM NEAA (100X) Gibco Cat #11140-050 Na Pyruvate (100mM) Gibco Cat #11360-070 Penicillin/Streptomycin Gibco Cat #15140-122 Golgi Stop BD Biosciences Cat #554724 Golgi Plug BD Biosciences Cat #555029 Human Trustain FcX BioLegend Cat #422302 Cell Acquisition Solution Fluidigm Cat #201240 CD69 RNAScope Probe on Channel 2 ACD Bio Cat # 494471-C2 CD3 RNAScope Probe on Channel 1 ACD Bio Cat #553971-C1 Ionomycin Calcium Salt from Streptomyces conglobatus Sigma-Aldrich Cat #I0634 Phorbol 12-Myristate 13-Acetate (PMA) Sigma-Aldrich Cat #P8139 FoxP3 Fix and Perm Buffer A Thermo Fisher Scientific Cat #00-5123-43 FoxP3 Fix and Perm Buffer B Thermo Fisher Scientific Cat #00-5223-56 FoxP3 Wash Buffer Thermo Fisher Scientific Cat #A24261 Formaldehyde Solution Sigma-Aldrich Cat #F8775 DNase I Roche Cat #11284932001 Collagenase A Sigma-Aldrich Cat #10103586001 Fetal Bovine Serum Corning Cat #35-011-CV Cell-ID Intercalator-Ir Fluidigm Cat #201192A Maxpar Fix and Perm Buffer Fluidigm Cat #201067 Critical Commercial Assays RNAscope 2.5 HD Duplex Reagent Kit Advanced Cell Diagnostics Cat #322430 Wizard Genomic DNA Purification Kit Promega Cat #A1120 (Continued on next page) Developmental Cell 51, 1–17.e1–e5, November 4, 2019 e2 ..

    Recombinant:

    Article Title: Maturation of the Human Intestinal Immune System Occurs Early in Fetal Development.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CD11b-167Er; ICRF44 Fluidigm Cat #3167011B; RRID:AB_2810974 IL-17a-169Tm; BL168* BioLegend Cat #512331; RRID:AB_2563779 IL-8-170Er; 6217* R&D Systems Cat #MAB208; RRID:AB_2249110 IL-21-172Yb; 3A3-N2 Fluidigm Cat #3172011B; RRID:AB_2810975 IL-10-176Yb; JES3-19F1* BioLegend Cat #506802; RRID:AB_315452 CCR7-BV421; 150503 BD Biosciences Cat #562555; RRID:AB_2728119 CD3-PE-Cy7; SK7 BioLegend Cat #344816; RRID:AB_10640737 CD4-APC-H7; RPA-T4 BD Biosciences Cat #560158; RRID:AB_1645478 CD8-PerCP-Cy5.5; RPA-T8 eBioscience Cat #45-0088-41; RRID:AB_1582256 CD45RA-FITC; HI100 BD Biosciences Cat #555488; RRID:AB_395879 CD45-BV785; HI30 BioLegend Cat #304048; RRID:AB_2563129 CD69-PE; FN50 BioLegend Cat #310906; RRID:AB_314841 Biological Samples Pitt Biospecimen Core University of Pittsburgh http://www.pittbiospecimencore.pitt.edu/ Chemicals, Peptides, and Recombinant Proteins Low-Barium PBS Sigma-Aldrich Cat #D8357 Bovine Serum Albumin, Protease-Free Sigma-Aldrich Cat #A3059 Sodium Azide Sigma-Aldrich Cat #71289 Cell-ID Intercalator-103Rh Fluidigm Cat #201103A Dimethyl Sulfoxide (DMSO) Fisher Scientific Cat #BP231-1; CAS:67-68-5 RPMI (1X) Media Gibco Cat #11875-093 GlutaMAX (100X) Gibco Cat #35050-061 HEPES (1M) Gibco Cat #15630-080 MEM NEAA (100X) Gibco Cat #11140-050 Na Pyruvate (100mM) Gibco Cat #11360-070 Penicillin/Streptomycin Gibco Cat #15140-122 Golgi Stop BD Biosciences Cat #554724 Golgi Plug BD Biosciences Cat #555029 Human Trustain FcX BioLegend Cat #422302 Cell Acquisition Solution Fluidigm Cat #201240 CD69 RNAScope Probe on Channel 2 ACD Bio Cat # 494471-C2 CD3 RNAScope Probe on Channel 1 ACD Bio Cat #553971-C1 Ionomycin Calcium Salt from Streptomyces conglobatus Sigma-Aldrich Cat #I0634 Phorbol 12-Myristate 13-Acetate (PMA) Sigma-Aldrich Cat #P8139 FoxP3 Fix and Perm Buffer A Thermo Fisher Scientific Cat #00-5123-43 FoxP3 Fix and Perm Buffer B Thermo Fisher Scientific Cat #00-5223-56 FoxP3 Wash Buffer Thermo Fisher Scientific Cat #A24261 Formaldehyde Solution Sigma-Aldrich Cat #F8775 DNase I Roche Cat #11284932001 Collagenase A Sigma-Aldrich Cat #10103586001 Fetal Bovine Serum Corning Cat #35-011-CV Cell-ID Intercalator-Ir Fluidigm Cat #201192A Maxpar Fix and Perm Buffer Fluidigm Cat #201067 Critical Commercial Assays RNAscope 2.5 HD Duplex Reagent Kit Advanced Cell Diagnostics Cat #322430 Wizard Genomic DNA Purification Kit Promega Cat #A1120 (Continued on next page) Developmental Cell 51, 1–17.e1–e5, November 4, 2019 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER CD11b-167Er; ICRF44 Fluidigm Cat #3167011B; RRID:AB_2810974 IL-17a-169Tm; BL168* BioLegend Cat #512331; RRID:AB_2563779 IL-8-170Er; 6217* R&D Systems Cat #MAB208; RRID:AB_2249110 IL-21-172Yb; 3A3-N2 Fluidigm Cat #3172011B; RRID:AB_2810975 IL-10-176Yb; JES3-19F1* BioLegend Cat #506802; RRID:AB_315452 CCR7-BV421; 150503 BD Biosciences Cat #562555; RRID:AB_2728119 CD3-PE-Cy7; SK7 BioLegend Cat #344816; RRID:AB_10640737 CD4-APC-H7; RPA-T4 BD Biosciences Cat #560158; RRID:AB_1645478 CD8-PerCP-Cy5.5; RPA-T8 eBioscience Cat #45-0088-41; RRID:AB_1582256 CD45RA-FITC; HI100 BD Biosciences Cat #555488; RRID:AB_395879 CD45-BV785; HI30 BioLegend Cat #304048; RRID:AB_2563129 CD69-PE; FN50 BioLegend Cat #310906; RRID:AB_314841 Biological Samples Pitt Biospecimen Core University of Pittsburgh http://www.pittbiospecimencore.pitt.edu/ Chemicals, Peptides, and Recombinant Proteins Low-Barium PBS Sigma-Aldrich Cat #D8357 Bovine Serum Albumin, Protease-Free Sigma-Aldrich Cat #A3059 Sodium Azide Sigma-Aldrich Cat #71289 Cell-ID Intercalator-103Rh Fluidigm Cat #201103A Dimethyl Sulfoxide (DMSO) Fisher Scientific Cat #BP231-1; CAS:67-68-5 RPMI (1X) Media Gibco Cat #11875-093 GlutaMAX (100X) Gibco Cat #35050-061 HEPES (1M) Gibco Cat #15630-080 MEM NEAA (100X) Gibco Cat #11140-050 Na Pyruvate (100mM) Gibco Cat #11360-070 Penicillin/Streptomycin Gibco Cat #15140-122 Golgi Stop BD Biosciences Cat #554724 Golgi Plug BD Biosciences Cat #555029 Human Trustain FcX BioLegend Cat #422302 Cell Acquisition Solution Fluidigm Cat #201240 CD69 RNAScope Probe on Channel 2 ACD Bio Cat # 494471-C2 CD3 RNAScope Probe on Channel 1 ACD Bio Cat #553971-C1 Ionomycin Calcium Salt from Streptomyces conglobatus Sigma-Aldrich Cat #I0634 Phorbol 12-Myristate 13-Acetate (PMA) Sigma-Aldrich Cat #P8139 FoxP3 Fix and Perm Buffer A Thermo Fisher Scientific Cat #00-5123-43 FoxP3 Fix and Perm Buffer B Thermo Fisher Scientific Cat #00-5223-56 FoxP3 Wash Buffer Thermo Fisher Scientific Cat #A24261 Formaldehyde Solution Sigma-Aldrich Cat #F8775 DNase I Roche Cat #11284932001 Collagenase A Sigma-Aldrich Cat #10103586001 Fetal Bovine Serum Corning Cat #35-011-CV Cell-ID Intercalator-Ir Fluidigm Cat #201192A Maxpar Fix and Perm Buffer Fluidigm Cat #201067 Critical Commercial Assays RNAscope 2.5 HD Duplex Reagent Kit Advanced Cell Diagnostics Cat #322430 Wizard Genomic DNA Purification Kit Promega Cat #A1120 (Continued on next page) Developmental Cell 51, 1–17.e1–e5, November 4, 2019 e2 ..

    RNAscope:

    Article Title: Maturation of the Human Intestinal Immune System Occurs Early in Fetal Development.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CD11b-167Er; ICRF44 Fluidigm Cat #3167011B; RRID:AB_2810974 IL-17a-169Tm; BL168* BioLegend Cat #512331; RRID:AB_2563779 IL-8-170Er; 6217* R&D Systems Cat #MAB208; RRID:AB_2249110 IL-21-172Yb; 3A3-N2 Fluidigm Cat #3172011B; RRID:AB_2810975 IL-10-176Yb; JES3-19F1* BioLegend Cat #506802; RRID:AB_315452 CCR7-BV421; 150503 BD Biosciences Cat #562555; RRID:AB_2728119 CD3-PE-Cy7; SK7 BioLegend Cat #344816; RRID:AB_10640737 CD4-APC-H7; RPA-T4 BD Biosciences Cat #560158; RRID:AB_1645478 CD8-PerCP-Cy5.5; RPA-T8 eBioscience Cat #45-0088-41; RRID:AB_1582256 CD45RA-FITC; HI100 BD Biosciences Cat #555488; RRID:AB_395879 CD45-BV785; HI30 BioLegend Cat #304048; RRID:AB_2563129 CD69-PE; FN50 BioLegend Cat #310906; RRID:AB_314841 Biological Samples Pitt Biospecimen Core University of Pittsburgh http://www.pittbiospecimencore.pitt.edu/ Chemicals, Peptides, and Recombinant Proteins Low-Barium PBS Sigma-Aldrich Cat #D8357 Bovine Serum Albumin, Protease-Free Sigma-Aldrich Cat #A3059 Sodium Azide Sigma-Aldrich Cat #71289 Cell-ID Intercalator-103Rh Fluidigm Cat #201103A Dimethyl Sulfoxide (DMSO) Fisher Scientific Cat #BP231-1; CAS:67-68-5 RPMI (1X) Media Gibco Cat #11875-093 GlutaMAX (100X) Gibco Cat #35050-061 HEPES (1M) Gibco Cat #15630-080 MEM NEAA (100X) Gibco Cat #11140-050 Na Pyruvate (100mM) Gibco Cat #11360-070 Penicillin/Streptomycin Gibco Cat #15140-122 Golgi Stop BD Biosciences Cat #554724 Golgi Plug BD Biosciences Cat #555029 Human Trustain FcX BioLegend Cat #422302 Cell Acquisition Solution Fluidigm Cat #201240 CD69 RNAScope Probe on Channel 2 ACD Bio Cat # 494471-C2 CD3 RNAScope Probe on Channel 1 ACD Bio Cat #553971-C1 Ionomycin Calcium Salt from Streptomyces conglobatus Sigma-Aldrich Cat #I0634 Phorbol 12-Myristate 13-Acetate (PMA) Sigma-Aldrich Cat #P8139 FoxP3 Fix and Perm Buffer A Thermo Fisher Scientific Cat #00-5123-43 FoxP3 Fix and Perm Buffer B Thermo Fisher Scientific Cat #00-5223-56 FoxP3 Wash Buffer Thermo Fisher Scientific Cat #A24261 Formaldehyde Solution Sigma-Aldrich Cat #F8775 DNase I Roche Cat #11284932001 Collagenase A Sigma-Aldrich Cat #10103586001 Fetal Bovine Serum Corning Cat #35-011-CV Cell-ID Intercalator-Ir Fluidigm Cat #201192A Maxpar Fix and Perm Buffer Fluidigm Cat #201067 Critical Commercial Assays RNAscope 2.5 HD Duplex Reagent Kit Advanced Cell Diagnostics Cat #322430 Wizard Genomic DNA Purification Kit Promega Cat #A1120 (Continued on next page) Developmental Cell 51, 1–17.e1–e5, November 4, 2019 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER CD11b-167Er; ICRF44 Fluidigm Cat #3167011B; RRID:AB_2810974 IL-17a-169Tm; BL168* BioLegend Cat #512331; RRID:AB_2563779 IL-8-170Er; 6217* R&D Systems Cat #MAB208; RRID:AB_2249110 IL-21-172Yb; 3A3-N2 Fluidigm Cat #3172011B; RRID:AB_2810975 IL-10-176Yb; JES3-19F1* BioLegend Cat #506802; RRID:AB_315452 CCR7-BV421; 150503 BD Biosciences Cat #562555; RRID:AB_2728119 CD3-PE-Cy7; SK7 BioLegend Cat #344816; RRID:AB_10640737 CD4-APC-H7; RPA-T4 BD Biosciences Cat #560158; RRID:AB_1645478 CD8-PerCP-Cy5.5; RPA-T8 eBioscience Cat #45-0088-41; RRID:AB_1582256 CD45RA-FITC; HI100 BD Biosciences Cat #555488; RRID:AB_395879 CD45-BV785; HI30 BioLegend Cat #304048; RRID:AB_2563129 CD69-PE; FN50 BioLegend Cat #310906; RRID:AB_314841 Biological Samples Pitt Biospecimen Core University of Pittsburgh http://www.pittbiospecimencore.pitt.edu/ Chemicals, Peptides, and Recombinant Proteins Low-Barium PBS Sigma-Aldrich Cat #D8357 Bovine Serum Albumin, Protease-Free Sigma-Aldrich Cat #A3059 Sodium Azide Sigma-Aldrich Cat #71289 Cell-ID Intercalator-103Rh Fluidigm Cat #201103A Dimethyl Sulfoxide (DMSO) Fisher Scientific Cat #BP231-1; CAS:67-68-5 RPMI (1X) Media Gibco Cat #11875-093 GlutaMAX (100X) Gibco Cat #35050-061 HEPES (1M) Gibco Cat #15630-080 MEM NEAA (100X) Gibco Cat #11140-050 Na Pyruvate (100mM) Gibco Cat #11360-070 Penicillin/Streptomycin Gibco Cat #15140-122 Golgi Stop BD Biosciences Cat #554724 Golgi Plug BD Biosciences Cat #555029 Human Trustain FcX BioLegend Cat #422302 Cell Acquisition Solution Fluidigm Cat #201240 CD69 RNAScope Probe on Channel 2 ACD Bio Cat # 494471-C2 CD3 RNAScope Probe on Channel 1 ACD Bio Cat #553971-C1 Ionomycin Calcium Salt from Streptomyces conglobatus Sigma-Aldrich Cat #I0634 Phorbol 12-Myristate 13-Acetate (PMA) Sigma-Aldrich Cat #P8139 FoxP3 Fix and Perm Buffer A Thermo Fisher Scientific Cat #00-5123-43 FoxP3 Fix and Perm Buffer B Thermo Fisher Scientific Cat #00-5223-56 FoxP3 Wash Buffer Thermo Fisher Scientific Cat #A24261 Formaldehyde Solution Sigma-Aldrich Cat #F8775 DNase I Roche Cat #11284932001 Collagenase A Sigma-Aldrich Cat #10103586001 Fetal Bovine Serum Corning Cat #35-011-CV Cell-ID Intercalator-Ir Fluidigm Cat #201192A Maxpar Fix and Perm Buffer Fluidigm Cat #201067 Critical Commercial Assays RNAscope 2.5 HD Duplex Reagent Kit Advanced Cell Diagnostics Cat #322430 Wizard Genomic DNA Purification Kit Promega Cat #A1120 (Continued on next page) Developmental Cell 51, 1–17.e1–e5, November 4, 2019 e2 ..

    DNA Purification:

    Article Title: Maturation of the Human Intestinal Immune System Occurs Early in Fetal Development.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CD11b-167Er; ICRF44 Fluidigm Cat #3167011B; RRID:AB_2810974 IL-17a-169Tm; BL168* BioLegend Cat #512331; RRID:AB_2563779 IL-8-170Er; 6217* R&D Systems Cat #MAB208; RRID:AB_2249110 IL-21-172Yb; 3A3-N2 Fluidigm Cat #3172011B; RRID:AB_2810975 IL-10-176Yb; JES3-19F1* BioLegend Cat #506802; RRID:AB_315452 CCR7-BV421; 150503 BD Biosciences Cat #562555; RRID:AB_2728119 CD3-PE-Cy7; SK7 BioLegend Cat #344816; RRID:AB_10640737 CD4-APC-H7; RPA-T4 BD Biosciences Cat #560158; RRID:AB_1645478 CD8-PerCP-Cy5.5; RPA-T8 eBioscience Cat #45-0088-41; RRID:AB_1582256 CD45RA-FITC; HI100 BD Biosciences Cat #555488; RRID:AB_395879 CD45-BV785; HI30 BioLegend Cat #304048; RRID:AB_2563129 CD69-PE; FN50 BioLegend Cat #310906; RRID:AB_314841 Biological Samples Pitt Biospecimen Core University of Pittsburgh http://www.pittbiospecimencore.pitt.edu/ Chemicals, Peptides, and Recombinant Proteins Low-Barium PBS Sigma-Aldrich Cat #D8357 Bovine Serum Albumin, Protease-Free Sigma-Aldrich Cat #A3059 Sodium Azide Sigma-Aldrich Cat #71289 Cell-ID Intercalator-103Rh Fluidigm Cat #201103A Dimethyl Sulfoxide (DMSO) Fisher Scientific Cat #BP231-1; CAS:67-68-5 RPMI (1X) Media Gibco Cat #11875-093 GlutaMAX (100X) Gibco Cat #35050-061 HEPES (1M) Gibco Cat #15630-080 MEM NEAA (100X) Gibco Cat #11140-050 Na Pyruvate (100mM) Gibco Cat #11360-070 Penicillin/Streptomycin Gibco Cat #15140-122 Golgi Stop BD Biosciences Cat #554724 Golgi Plug BD Biosciences Cat #555029 Human Trustain FcX BioLegend Cat #422302 Cell Acquisition Solution Fluidigm Cat #201240 CD69 RNAScope Probe on Channel 2 ACD Bio Cat # 494471-C2 CD3 RNAScope Probe on Channel 1 ACD Bio Cat #553971-C1 Ionomycin Calcium Salt from Streptomyces conglobatus Sigma-Aldrich Cat #I0634 Phorbol 12-Myristate 13-Acetate (PMA) Sigma-Aldrich Cat #P8139 FoxP3 Fix and Perm Buffer A Thermo Fisher Scientific Cat #00-5123-43 FoxP3 Fix and Perm Buffer B Thermo Fisher Scientific Cat #00-5223-56 FoxP3 Wash Buffer Thermo Fisher Scientific Cat #A24261 Formaldehyde Solution Sigma-Aldrich Cat #F8775 DNase I Roche Cat #11284932001 Collagenase A Sigma-Aldrich Cat #10103586001 Fetal Bovine Serum Corning Cat #35-011-CV Cell-ID Intercalator-Ir Fluidigm Cat #201192A Maxpar Fix and Perm Buffer Fluidigm Cat #201067 Critical Commercial Assays RNAscope 2.5 HD Duplex Reagent Kit Advanced Cell Diagnostics Cat #322430 Wizard Genomic DNA Purification Kit Promega Cat #A1120 (Continued on next page) Developmental Cell 51, 1–17.e1–e5, November 4, 2019 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER CD11b-167Er; ICRF44 Fluidigm Cat #3167011B; RRID:AB_2810974 IL-17a-169Tm; BL168* BioLegend Cat #512331; RRID:AB_2563779 IL-8-170Er; 6217* R&D Systems Cat #MAB208; RRID:AB_2249110 IL-21-172Yb; 3A3-N2 Fluidigm Cat #3172011B; RRID:AB_2810975 IL-10-176Yb; JES3-19F1* BioLegend Cat #506802; RRID:AB_315452 CCR7-BV421; 150503 BD Biosciences Cat #562555; RRID:AB_2728119 CD3-PE-Cy7; SK7 BioLegend Cat #344816; RRID:AB_10640737 CD4-APC-H7; RPA-T4 BD Biosciences Cat #560158; RRID:AB_1645478 CD8-PerCP-Cy5.5; RPA-T8 eBioscience Cat #45-0088-41; RRID:AB_1582256 CD45RA-FITC; HI100 BD Biosciences Cat #555488; RRID:AB_395879 CD45-BV785; HI30 BioLegend Cat #304048; RRID:AB_2563129 CD69-PE; FN50 BioLegend Cat #310906; RRID:AB_314841 Biological Samples Pitt Biospecimen Core University of Pittsburgh http://www.pittbiospecimencore.pitt.edu/ Chemicals, Peptides, and Recombinant Proteins Low-Barium PBS Sigma-Aldrich Cat #D8357 Bovine Serum Albumin, Protease-Free Sigma-Aldrich Cat #A3059 Sodium Azide Sigma-Aldrich Cat #71289 Cell-ID Intercalator-103Rh Fluidigm Cat #201103A Dimethyl Sulfoxide (DMSO) Fisher Scientific Cat #BP231-1; CAS:67-68-5 RPMI (1X) Media Gibco Cat #11875-093 GlutaMAX (100X) Gibco Cat #35050-061 HEPES (1M) Gibco Cat #15630-080 MEM NEAA (100X) Gibco Cat #11140-050 Na Pyruvate (100mM) Gibco Cat #11360-070 Penicillin/Streptomycin Gibco Cat #15140-122 Golgi Stop BD Biosciences Cat #554724 Golgi Plug BD Biosciences Cat #555029 Human Trustain FcX BioLegend Cat #422302 Cell Acquisition Solution Fluidigm Cat #201240 CD69 RNAScope Probe on Channel 2 ACD Bio Cat # 494471-C2 CD3 RNAScope Probe on Channel 1 ACD Bio Cat #553971-C1 Ionomycin Calcium Salt from Streptomyces conglobatus Sigma-Aldrich Cat #I0634 Phorbol 12-Myristate 13-Acetate (PMA) Sigma-Aldrich Cat #P8139 FoxP3 Fix and Perm Buffer A Thermo Fisher Scientific Cat #00-5123-43 FoxP3 Fix and Perm Buffer B Thermo Fisher Scientific Cat #00-5223-56 FoxP3 Wash Buffer Thermo Fisher Scientific Cat #A24261 Formaldehyde Solution Sigma-Aldrich Cat #F8775 DNase I Roche Cat #11284932001 Collagenase A Sigma-Aldrich Cat #10103586001 Fetal Bovine Serum Corning Cat #35-011-CV Cell-ID Intercalator-Ir Fluidigm Cat #201192A Maxpar Fix and Perm Buffer Fluidigm Cat #201067 Critical Commercial Assays RNAscope 2.5 HD Duplex Reagent Kit Advanced Cell Diagnostics Cat #322430 Wizard Genomic DNA Purification Kit Promega Cat #A1120 (Continued on next page) Developmental Cell 51, 1–17.e1–e5, November 4, 2019 e2 ..



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